mouse anti gad67  (Proteintech)

Journal: Translational Psychiatry

Article Title: Deletion of Fgf14 confers resilience to basal and stress-induced depressive-like behavior and reduces anxiety in mice

doi: 10.1038/s41398-025-03361-z

Figure Lengend Snippet: A Representative images of the coronal sections of mPFC of WT and Fgf14 −/− mice under basal conditions or following acute stress induced by TST immunostained with cFOS (red) and GAD67 (white) antibody. cFOS + cell density was evaluated in the non-GABAergic (GAD67 - ) and GABAergic (GAD67 + ) cells of whole mPFC in WT B and Fgf14 −/− mice C under basal conditions or following acute stress induced by TST. The analysis reveals that the majority of cFOS + cells is non-GABAergic, and that the amount of such cells is significantly higher after TST only in Fgf14 −/− mice. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: To detect cFOS positivity in GAD67 positive ( + ) GABAergic neurons, slices were incubated overnight at 4 °C with the polyclonal anti-rabbit cFOS (9F6, Cell Signaling Technology, MA, USA) and with the monoclonal anti-mouse GAD67 (Merck-Millipore, Cat# MAB5406, RRID: AB_2278725) diluted (1:1000) in PBS with 0.25% Triton ×-100 and 1.5% normal donkey serum.

Techniques: